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Antibodies sure to the blocking peptide not bind to your epitope about the goal protein. By evaluating the staining from your blocked antibody vs . the antibody by yourself, you can see which staining is specific.

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This guideline outlines more probable optimization of cellular and immunoassay parameters to be certain the very best final results are...

This information outlines further feasible optimization of cellular and immunoassay parameters to be sure the very best success are...

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If an Invitrogen™ antibody doesn't conduct as described on our website or datasheet,we will replace the products without charge to you personally, or offer you a credit for the future invest in.*

The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Clicking the pictures or backlinks will redirect you to an internet site hosted by BenchSci that provides 3rd-social gathering scientific material. Neither the content nor the BenchSci technology and processes for selection are evaluated by us; we're furnishing them as-is and without having guarantee of any type, which include for use or application with the Thermo Fisher Scientific goods offered.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...

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Blocking peptides are peptides that bind precisely towards the target antibody and block antibody binding. These peptide ordinarily incorporates the epitope identified through the antibody.

Target Information and facts Cyclin E belongs to your very conserved cyclin loved ones, whose users are characterized by a extraordinary periodicity in protein abundance throughout the cell cycle. Cyclins operate as regulators of check here CDK kinases. Various cyclins show distinctive expression and degradation designs which add towards the temporal coordination of each and every mitotic event. Cyclin E varieties a complex with and features for a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S changeover. This protein accumulates in the G1-S period boundary and is particularly degraded as cells progress by means of S period.

Overexpression of the gene continues to be observed in lots of tumors, which leads to chromosome instability, and therefore may possibly lead to tumorigenesis. This protein was observed to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a important purpose in advertising cell-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have been explained. Two more splice variants ended up described but specific nucleotide sequence data just isn't however accessible.

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